Type 1 diabetes (T1D) is increasing around the world due to unknown changes in our lifestyle. One factor that may drive this change is the bacteria in our intestines. These bacteria interact potently with our immune system and metabolism. We have shown that changes in intestinal and pancreatic proteins found in stool are associated with risk of T1D development. By integrating these data with microbial community profiling, we have shown that host proteins linked to gut barrier function and exocrine pancreas function are associated with changes in specific gut microbes. Now we plan to investigate whether changes in proteins found in stoolare associated with inflammation present in either the intestine or pancreas of individuals with islet autoimmunity.