nPOD Standard Operating Procedures

The nPOD Standard Operating Procedures are available to all nPOD investigators and partners in PDF format. These procedures are approved by the nPOD administrative core. A brief description of each procedure or protocol is provided for your convenience below. If you have any questions about any of the information included in these documents, please contact the nPOD administrative core.

 

Standard Operating Procedures by Category
Recovery Related SOP
Case Processing Related SOP
Lab Analysis SOP

 

Recovery Related Standard Operating Procedures:

Inclusion and Exclusion Criteria for nPOD Donors PDF

The purpose of this Standard Operating Procedure (SOP) is to define the current nPOD donor groups and
provide acceptance inclusion and exclusion criteria for each group.

Organ Packaging Instructions PDF

The purpose of this instruction is to provide a visual of how organ and tissues should be prepared in a package for shipping.

Organ Procurement Organization Recovery Instructions

If you are looking for nPOD organ recovery instructions, please visit the OPO Recovery page of our website. (NOTE: You will need a username and password to view these documents, and are only provided one if you are a member of a nPOD partner OPO.)

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Case Processing Related Standard Operating Procedures:

Case Processing PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for processing and
storing pancreas and other organs or tissues including serum and whole blood by the nPOD Organ
Processing and Pathology Core (OPPC).

Link to video of processes.

Histology PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for histological preparation of nPOD samples.

Immunopathology PDF

The purpose of this Standard Operating Procedure (SOP) is to outline the procedures for immunopathology preparation and analysis of nPOD samples.

Link to video of these processes.

Isolation of PBMC PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for isolation and
cryopreservation of peripheral blood mononuclear cells (PBMC).

Isolation of Cells from Spleen and Lymph Nodes PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for isolating and
cryopreserving cells from spleen, thymus, and lymph nodes.

DNA Extraction PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for extracting high
quality genomic DNA from nPOD samples.

RNA Extraction PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for extracting high quality RNA from nPOD samples.

Cryopreserved Cell Thawing PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for thawing cryopreserved
cells prepared by the nPOD OPPC.

Sample Distributions PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for shipping samples to
nPOD investigators from the OPPC.

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Lab Analysis Standard Operating Procedures:

IA-2 Antibody Protocol PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for using the modified
KRONUS® IA-2 Autoantibody ELISA kit.

GAD Antibody Protocol PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for using the modified
KRONUS® GAD Autoantibody ELISA kit.

Shipping from Autoantibody Screening Laboratories PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for labeling, storing,
and shipping autoantibody positive samples from screening laboratories to nPOD administrative offices
at the University of Florida.

Linear Array Genotyping System for HLA PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for genotyping nPOD
donor cases based on human leukocyte antigen (HLA).

C-peptide Determination PDF

The purpose of this Standard Operating Procedure (SOP) is to outline procedures for preparing and
shipping serum from nPOD donors for C-peptide measurement.

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