nPOD Standard Operating Procedures

The nPOD Standard Operating Procedures are available to all nPOD investigators and partners in PDF format. These procedures are approved by the nPOD executive committee. A brief description of each procedure or protocol is provided for your convenience below. If you have any questions about any of the information included in these documents, please contact the nPOD administrative core.

nPOD1: Shipping and Handling Procedures PDF

The purpose of this document is to serve as a guide by which donor tissue, including pancreas, spleen, duodenum, and peripheral blood samples, are shipped to the nPOD Organ Procurement and Pathology Core (OPPC) from National Disease Resource Interchange (NDRI) or Organ Procurement Organizations. It will also serve as a guide for shipping and delivery of tissue to nPOD investigators from the OPPC.

nPOD2: Inclusion and Exclusion Criteria for nPOD Donors PDF

The purpose of this document is to define the current nPOD donor groups and provide the inclusion and exclusion criteria for each group.

nPOD3: Organ Processing and Histopathology PDF

The purpose of this document is to define the processes and procedures undertaken by nPOD's Organ Procurement and Pathology Core (OPCC).

nPOD5: C-peptide Determination PDF

The purpose of this document is to define the processes and procedures for CPeptide measurement as they are performed for the JDRF nPOD project.

nPOD6: IA-2 Antibody Protocol PDF

The purpose of this document is to define the protocol for the use of the modified KRONUS®, IA-2 Autoantibody ELISA kit.

nPOD7: GAD Antibody Protocol PDF

The purpose of this document is to define the protocol for the use of the modified KRONUS®, GAD Autoantibody ELISA kit.

nPOD8: Introduction to the Linear Array Genotyping System PDF

The purpose of this document is to define the processes and procedures for nPOD project genotyping.

nPOD9: Isolation of Cells from Spleen and Lymph Nodes PDF

The purpose of this procedure is to document the process by which cells from spleen and lymph nodes are isolated and frozen.

nPOD10: DNA Extraction PDF

The purpose of this procedure is to establish guidelines to isolate Genomic DNA from human tissue.

nPOD11: Isolation of PBMC PDF

The purpose of this procedure is to document the process by which peripheral blood mononuclear cells (PBMC) are isolated and frozen.

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AAb1: Shipping: Positive Samples (LABS, Inc., Denver) PDF

The purpose of this document is to define the process for storing, shipping, and labeling autoantibody positive samples from LABS, Inc. in Denver to the nPOD administrative offices at the University of Florida.

AAb2: Shipping: Positive Samples (MNIT) PDF

The purpose of this document is to define the process for storing, shipping, and labeling autoantibody positive samples from MNIT in Los Angeles to the nPOD administrative offices at the University of Florida.

AAb3: Shipping: Positive Samples (University of Miami nPOD Screening Lab) PDF

The purpose of this document is to define the process for storing, shipping, and labeling autoantibody positive samples from the nPOD screening lab at the University of Miami to the nPOD administrative offices at the University of Florida.

AAb4: Shipping: Positive Samples (LABS, Inc., Philadelphia) PDF

The purpose of this document is to define the process for storing, shipping, and labeling autoantibody positive samples from LABS, Inc. in Philadelphia to the nPOD administrative offices at the University of Florida.