Request nPOD Samples

If you are an approved nPOD investigator and would like to request slide and/or tissue samples, please contact Irina, the OPPC Lab Manager, regarding available inventory (especially cell requests), before you fill out and submit the nPOD Service Request Form.

Note: Because of its reserved status, requests for nPOD Case 6052 will always require further justification.

 

For help filling out the service request form, click on this image:

 

• Please note: the maximum number of sections per block per request is 10. Please confirm that you have requested the minimum number of slides per block required to complete your assays. The slides will be provided as numbered serial sections. We request that nPOD slides are not used as assay positive and negative slides, rather other available control donor blocks be used for assay controls. All these efforts for minimizing slides per block are based on the need to conserve this precious resource. We further request that investigators use multiple labeling with received slides.

Investigator FAQ's

Other Investigator Services

These services are provided by the Organ Processing and Pathology Core (OPPC).

nPOD provides tissue recovery services for pancreata and related tissues from autoantibody positive donors and donors with type 1 diabetes. Tissue from donors who do not have type 1 diabetes is also recovered to provide critical controls for the autoantibody positive and type 1 diabetes samples.

Collected tissues are:

• Pancreas (head, body and tail)
• Pancreatic lymph nodes
• Spleen
• Non-pancreatic lymph nodes

Note: All tissue types may not be available for every case. Please consult OPPC for sample availability.

Samples are recovered into UW solution and transported to the organ procurement and pathology core via tissue donor courier services.

Upon receipt, tissues are dissected for:

• NBF fixation and paraffin processing
• Fresh frozen OCT blocks
• Lymphocytes and Splenocytes
• PBMCs
• Snap frozen samples in vials to be maintained at -80
• Additional procedures as required by JDRF investigators
  

In addition:

• The cell concentration is 1X10^7/ml and aliquot volume may vary between 0.2-1.5ml, depending on each individual case. The OPPC lab can confirm availability.
• Quality control reviews are performed by examination of fixed samples with H&E and IHC staining
• Histology and IHC services are available through the Molecular Pathology Core

All Institutional and Federal requirements for human tissue banking are current.

• IRB approval letter

nPOD relies on its partnership with the Molecular Pathology and Immunology Core at the University of Florida to provide its services to affiliated investigators.

Investigator FAQ's